PrimePCR™ Probe Assay: RFX1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RFX1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RFX1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028064
Assay Design:   Intron-spanning
Chromosome Location:   19:14083845-14088862question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000254325

Gene Information

This gene is a member of the regulatory factor X gene family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2 X3 X4 and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2 X3 and X5 but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. [provided by RefSeq Jul 2008]

Gene Symbol:   RFX1
Gene Name:   regulatory factor X, 1 (influences HLA class II expression)
Aliases:   EFC, RFX
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000132005
Entrez:   5989
Chromosome Mapping:   19p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.110000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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