PrimePCR™ Probe Assay: ACAD8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ACAD8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACAD8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028187
Assay Design:   Intron-spanning
Chromosome Location:   11:134128902-134129550question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000281182 ENST00000374752 ENST00000537915 ENST00000543332 ENST00000537423

Gene Information

This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The encoded protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency.[provided by RefSeq Nov 2009]

Gene Symbol:   ACAD8
Gene Name:   acyl-CoA dehydrogenase family, member 8
Aliases:   ACAD-8, ARC42, FLJ22590
RefSeq:   NC_000011.9 NG_015842.1 NT_033899.8
Ensembl:   ENSG00000151498
Entrez:   27034
Chromosome Mapping:   11q25

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