PrimePCR™ Probe Assay: MYOM2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYOM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYOM2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028245
Assay Design:   Intron-spanning
Chromosome Location:   8:2063865-2071134question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000262113 ENST00000523438

Gene Information

The giant protein titin together with its associated proteins interconnects the major structure of sarcomeres the M bands and Z discs. The C-terminal end of the titin string extends into the M line where it binds tightly to M-band constituents of apparent molecular masses of 190 kD and 165 kD. The predicted MYOM2 protein contains 1465 amino acids. Like MYOM1 MYOM2 has a unique N-terminal domain followed by 12 repeat domains with strong homology to either fibronectin type III or immunoglobulin C2 domains. Protein sequence comparisons suggested that the MYOM2 protein and bovine M protein are identical. [provided by RefSeq Jul 2008]

Gene Symbol:   MYOM2
Gene Name:   myomesin (M-protein) 2, 165kDa
Aliases:   TTNAP
RefSeq:   NC_000008.10 NT_023736.17
Ensembl:   ENSG00000036448
Entrez:   9172
Chromosome Mapping:   8p23.3

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