PrimePCR™ Probe Assay: FBXL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FBXL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FBXL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028279
Assay Design:   Intron-spanning
Chromosome Location:   3:33419595-33420230question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000484457 ENST00000507198 ENST00000538892 ENST00000538181 ENST00000446237 ENST00000542085

Gene Information

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box) which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains Fbls containing leucine-rich repeats and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and in addition to an F-box contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2010]

Gene Symbol:   FBXL2
Gene Name:   F-box and leucine-rich repeat protein 2
Aliases:   DKFZp564P0622, FBL2, FBL3
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000153558
Entrez:   25827
Chromosome Mapping:   3p22.3

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