PrimePCR™ Probe Assay: NR5A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NR5A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NR5A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028304
Assay Design:   Intron-spanning
Chromosome Location:   9:127255326-127262394question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000373588 ENST00000373587

Gene Information

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq Jul 2008]

Gene Symbol:   NR5A1
Gene Name:   nuclear receptor subfamily 5, group A, member 1
Aliases:   AD4BP, ELP, FTZ1, FTZF1, POF7, SF-1, SF1
RefSeq:   NC_000009.11 NG_008176.1 NT_008470.19
Ensembl:   ENSG00000136931
Entrez:   2516
Chromosome Mapping:   9q33

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