PrimePCR™ Probe Assay: HTR4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HTR4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HTR4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028308
Assay Design:   Intron-spanning
Chromosome Location:   5:147889504-147902798question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000521530 ENST00000521735 ENST00000517929 ENST00000520514 ENST00000377888 ENST00000360693 ENST00000354217 ENST00000314512 ENST00000362016

Gene Information

This gene is a member of the family of serotonin receptors which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq May 2010]

Gene Symbol:   HTR4
Gene Name:   5-hydroxytryptamine (serotonin) receptor 4
Aliases:   5-HT4, 5-HT4R
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000164270
Entrez:   3360
Chromosome Mapping:   5q31-q33

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