PrimePCR™ Probe Assay: SCAP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SCAP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCAP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028364
Assay Design:   Intron-spanning
Chromosome Location:   3:47462453-47463985question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000265565 ENST00000339815 ENST00000360832 ENST00000441517 ENST00000545718 ENST00000383739

Gene Information

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. [provided by RefSeq Jul 2008]

Gene Symbol:   SCAP
Gene Name:   SREBF chaperone
Aliases:   KIAA0199
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000114650
Entrez:   22937
Chromosome Mapping:   3p21.31

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