PrimePCR™ Probe Assay: ST3GAL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ST3GAL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ST3GAL4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028458
Assay Design:   Intron-spanning
Chromosome Location:   11:126279271-126283527question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000227495 ENST00000444328 ENST00000530591 ENST00000534083 ENST00000392669 ENST00000526727 ENST00000449406 ENST00000532243 ENST00000534457 ENST00000524860 ENST00000356132

Gene Information

This gene encodes a member of the glycosyltransferase 29 family a group of enzymes involved in protein glycosylation. The encoded protein is targeted to Golgi membranes but may be proteolytically processed and secreted. The gene product may also be involved in the increased expression of sialyl Lewis X antigen seen in inflammatory responses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   ST3GAL4
Gene Name:   ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Aliases:   CGS23, FLJ11867, FLJ46764, NANTA3, SAT3, SIAT4, SIAT4C, ST3GalIV, STZ
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000110080
Entrez:   6484
Chromosome Mapping:   11q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996300
y-intercept 35.380000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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