PrimePCR™ Probe Assay: APOA4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: APOA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: APOA4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028570
Assay Design:   Intron-spanning
Chromosome Location:   11:116692551-116693464question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000357780

Gene Information

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis the intestine in association with chylomicron particles. Although its precise function is not known apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq Jul 2008]

Gene Symbol:   APOA4
Gene Name:   apolipoprotein A-IV
Aliases:   MGC142154, MGC142156
RefSeq:   NC_000011.9 NG_012044.1 NT_033899.8
Ensembl:   ENSG00000110244
Entrez:   337
Chromosome Mapping:   11q23

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