PrimePCR™ Probe Assay: IGJ, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028585
Assay Design:   Intron-spanning
Chromosome Location:   4:71522131-71522979question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000254801 ENST00000510437 ENST00000510614 ENST00000391614 ENST00000543780

Gene Information

Description Not Available

Gene Symbol:   IGJ
Gene Name:   immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
Aliases:   IGCJ, JCH
RefSeq:   NC_000004.11 NT_022778.16
Ensembl:   ENSG00000132465
Entrez:   3512
Chromosome Mapping:   4q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.860000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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