PrimePCR™ Probe Assay: PCGF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PCGF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCGF1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029077
Assay Design:   Intron-spanning
Chromosome Location:   2:74733958-74734746question
Amplicon Length:   174
Splice Variants Targeted:   ENST00000233630

Gene Information

PCGF1 is a mammalian homolog of the Drosophila polycomb group genes which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al. 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM Aug 2008]

Gene Symbol:   PCGF1
Gene Name:   polycomb group ring finger 1
Aliases:   2010002K04Rik, FLJ43754, MGC10882, NSPC1, RNF3A-2, RNF68
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115289
Entrez:   84759
Chromosome Mapping:   2p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.720000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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