PrimePCR™ Probe Assay: COL5A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL5A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL5A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029421
Assay Design:   Intron-spanning
Chromosome Location:   9:137716573-137717731question
Amplicon Length:   193
Splice Variants Targeted:   ENST00000371817 ENST00000371820 ENST00000355306

Gene Information

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome types I and II. [provided by RefSeq Jul 2008]

Gene Symbol:   COL5A1
Gene Name:   collagen, type V, alpha 1
Aliases:   Not Available
RefSeq:   NC_000009.11 NG_008030.1 NT_019501.13
Ensembl:   ENSG00000130635
Entrez:   1289
Chromosome Mapping:   9q34.2-q34.3

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