PrimePCR™ Probe Assay: WNT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WNT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WNT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029498
Assay Design:   Intron-spanning
Chromosome Location:   12:49373463-49374311question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000293549

Gene Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member WNT10B in the chromosome 12q13 region. [provided by RefSeq Jul 2008]

Gene Symbol:   WNT1
Gene Name:   wingless-type MMTV integration site family, member 1
Aliases:   INT1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000125084
Entrez:   7471
Chromosome Mapping:   12q13

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