PrimePCR™ Probe Assay: SPTA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SPTA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPTA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029512
Assay Design:   Intron-spanning
Chromosome Location:   1:158632684-158636223question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000368148 ENST00000368147

Gene Information

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape arrangement of transmembrane proteins and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders including elliptocytosis type 2 pyropoikilocytosis and spherocytic hemolytic anemia. [provided by RefSeq Jul 2008]

Gene Symbol:   SPTA1
Gene Name:   spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
Aliases:   EL2, HPP, HS3, SPH3, SPTA
RefSeq:   NC_000001.10 NG_011474.1 NT_004487.19
Ensembl:   ENSG00000163554
Entrez:   6708
Chromosome Mapping:   1q21

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