PrimePCR™ Probe Assay: DDX6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DDX6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DDX6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029557
Assay Design:   Intron-spanning
Chromosome Location:   11:118630727-118635974question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000264018 ENST00000526070 ENST00000534980

Gene Information

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Mar 2012]

Gene Symbol:   DDX6
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Aliases:   FLJ36338, HLR2, P54, RCK
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000110367
Entrez:   1656
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.750000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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