PrimePCR™ Probe Assay: IKBKE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IKBKE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IKBKE, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029574
Assay Design:   Intron-spanning
Chromosome Location:   1:206664152-206666368question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000367120 ENST00000537984

Gene Information

IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al. 2009 [PubMed 19481526]).[supplied by OMIM Oct 2009]

Gene Symbol:   IKBKE
Gene Name:   inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Aliases:   IKK-E, IKK-i, IKKE, IKKI, KIAA0151, MGC125294, MGC125295, MGC125297
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143466
Entrez:   9641
Chromosome Mapping:   1q32.1

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