PrimePCR™ Probe Assay: COL17A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL17A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL17A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029613
Assay Design:   Intron-spanning
Chromosome Location:   10:105797394-105798199question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000353479 ENST00000369733 ENST00000541872

Gene Information

This gene encodes the alpha chain of type XVII collagen. Unlike most collagens collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form referred to as either ectodomain or LAD-1 is generated by proteolytic processing of the full length form. [provided by RefSeq Jul 2008]

Gene Symbol:   COL17A1
Gene Name:   collagen, type XVII, alpha 1
Aliases:   BA16H23.2, BP180, BPAG2, FLJ60881, KIAA0204, LAD-1
RefSeq:   NC_000010.10 NG_007069.1 NT_030059.13
Ensembl:   ENSG00000065618
Entrez:   1308
Chromosome Mapping:   10q24.3

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