PrimePCR™ Probe Assay: MAK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029851
Assay Design:   Intron-spanning
Chromosome Location:   6:10804015-10809105question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000313243 ENST00000354489 ENST00000538030 ENST00000536370

Gene Information

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is however a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2011]

Gene Symbol:   MAK
Gene Name:   male germ cell-associated kinase
Aliases:   dJ417M14.2
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000111837
Entrez:   4117
Chromosome Mapping:   6p24

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