PrimePCR™ Probe Assay: OBFC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: OBFC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: OBFC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029882
Assay Design:   Intron-spanning
Chromosome Location:   10:105648847-105652007question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000224950 ENST00000369764

Gene Information

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636) the enzyme that initiates DNA replication (Casteel et al. 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al. 2009 [PubMed 19854130]).[supplied by OMIM Nov 2009]

Gene Symbol:   OBFC1
Gene Name:   oligonucleotide/oligosaccharide-binding fold containing 1
Aliases:   AAF-44, AAF44, FLJ22559, RP11-541N10.2, RPA-32, STN1, bA541N10.2
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107960
Entrez:   79991
Chromosome Mapping:   10q24.33

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