PrimePCR™ Probe Assay: DNTT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DNTT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DNTT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029911
Assay Design:   Intron-spanning
Chromosome Location:   10:98095652-98097977question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000371174 ENST00000419175

Gene Information

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   DNTT
Gene Name:   deoxynucleotidyltransferase, terminal
Aliases:   TDT
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107447
Entrez:   1791
Chromosome Mapping:   10q23-q24

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