PrimePCR™ Probe Assay: ATP5G3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP5G3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP5G3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029998
Assay Design:   Intron-spanning
Chromosome Location:   2:176044838-176046143question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000284727 ENST00000409194 ENST00000392541

Gene Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core F1 and the membrane-spanning component Fo comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha beta gamma delta and epsilon) assembled with a stoichiometry of 3 alpha 3 beta and a single representative of the other 3. The proton channel seems to have nine subunits (a b c d e f g F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding different proteins have been identified. [provided by RefSeq Jun 2010]

Gene Symbol:   ATP5G3
Gene Name:   ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)
Aliases:   MGC125738, P3
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000154518
Entrez:   518
Chromosome Mapping:   2q31.1

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