PrimePCR™ Probe Assay: ATP6V0D1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP6V0D1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP6V0D1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030022
Assay Design:   Intron-spanning
Chromosome Location:   16:67473209-67477060question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000290949 ENST00000540149 ENST00000426604

Gene Information

This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits two G subunits plus the C D E F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a c c' c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP6V0D1
Gene Name:   ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Aliases:   ATP6D, ATP6DV, FLJ43534, P39, VATX, VMA6, VPATPD
RefSeq:   NC_000016.9 NG_011482.1 NG_011501.1 NG_016549.1 NT_010498.15
Ensembl:   ENSG00000159720
Entrez:   9114
Chromosome Mapping:   16q22.1

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