PrimePCR™ Probe Assay: ATP4B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP4B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP4B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030060
Assay Design:   Intron-spanning
Chromosome Location:   13:114307652-114309180question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000335288

Gene Information

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+ K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+ K+-ATPase. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP4B
Gene Name:   ATPase, H+/K+ exchanging, beta polypeptide
Aliases:   ATP6B
RefSeq:   NC_000013.10 NT_027140.6
Ensembl:   ENSG00000186009
Entrez:   496
Chromosome Mapping:   13q34

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