PrimePCR™ Probe Assay: SUCNR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SUCNR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SUCNR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030078
Assay Design:   Intron-spanning
Chromosome Location:   3:151591440-151597690question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000362032

Gene Information

This gene encodes a G-protein-coupled receptor for succinate an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development and it has a potential role in renovascular hypertension which has known correlations to renal failure diabetes and atherosclerosis. [provided by RefSeq Oct 2009]

Gene Symbol:   SUCNR1
Gene Name:   succinate receptor 1
Aliases:   GPR91
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000198829
Entrez:   56670
Chromosome Mapping:   3q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.900000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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