PrimePCR™ Probe Assay: MYBL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYBL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYBL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030189
Assay Design:   Intron-spanning
Chromosome Location:   20:42328624-42331171question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000217026 ENST00000396863

Gene Information

The protein encoded by this gene a member of the MYB family of transcription factor genes is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2 cyclin D1 and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene but their full-length natures have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   MYBL2
Gene Name:   v-myb myeloblastosis viral oncogene homolog (avian)-like 2
Aliases:   B-MYB, BMYB, MGC15600
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101057
Entrez:   4605
Chromosome Mapping:   20q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.240000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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