PrimePCR™ Probe Assay: GMPS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GMPS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GMPS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030199
Assay Design:   Intron-spanning
Chromosome Location:   3:155623989-155628539question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000496455 ENST00000295920 ENST00000541628 ENST00000537975

Gene Information

In the de novo synthesis of purine nucleotides IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway there are 2 enzymes involved in converting IMP to GMP namely IMP dehydrogenase (IMPD1) which catalyzes the oxidation of IMP to XMP and GMP synthetase which catalyzes the amination of XMP to GMP. [provided by RefSeq Jul 2008]

Gene Symbol:   GMPS
Gene Name:   guanine monphosphate synthetase
Aliases:   Not Available
RefSeq:   NC_000003.11 NG_023247.1 NT_005612.16
Ensembl:   ENSG00000163655
Entrez:   8833
Chromosome Mapping:   3q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.640000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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