PrimePCR™ Probe Assay: HNF4A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: HNF4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HNF4A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030230
Assay Design:   Intron-spanning
Chromosome Location:   20:43048473-43052751question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000316099 ENST00000443598 ENST00000457232 ENST00000338692 ENST00000415691 ENST00000316673

Gene Information

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes including hepatocyte nuclear factor 1 alpha a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver kidney and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq Jul 2008]

Gene Symbol:   HNF4A
Gene Name:   hepatocyte nuclear factor 4, alpha
Aliases:   FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14
RefSeq:   NC_000020.10 NG_009818.1 NT_011362.10
Ensembl:   ENSG00000101076
Entrez:   3172
Chromosome Mapping:   20q13.12

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