PrimePCR™ Probe Assay: PFKFB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PFKFB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PFKFB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030423
Assay Design:   Intron-spanning
Chromosome Location:   X:54960268-54964043question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000375006 ENST00000545676 ENST00000374992

Gene Information

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-26-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-26-biphosphate using independent catalytic domains. Fructose-26-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently regulating fructose-26-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq Jul 2008]

Gene Symbol:   PFKFB1
Gene Name:   6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Aliases:   F6PK, HL2K, MGC116715, MGC116717, PFRX
RefSeq:   NC_000023.10 NG_012806.1 NG_013254.1 NT_011630.14
Ensembl:   ENSG00000158571
Entrez:   5207
Chromosome Mapping:   Xp11.21

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