PrimePCR™ Probe Assay: TSLP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TSLP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TSLP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030468
Assay Design:   Intron-spanning
Chromosome Location:   5:110409252-110411727question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000420978 ENST00000344895 ENST00000379706

Gene Information

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases including asthma allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq Jan 2012]

Gene Symbol:   TSLP
Gene Name:   thymic stromal lymphopoietin
Aliases:   Not Available
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000145777
Entrez:   85480
Chromosome Mapping:   5q22.1

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