PrimePCR™ Probe Assay: MRPS10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MRPS10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MRPS10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030502
Assay Design:   Intron-spanning
Chromosome Location:   6:42179567-42181909question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000053468

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q 3p and 9p. [provided by RefSeq Jul 2008]

Gene Symbol:   MRPS10
Gene Name:   mitochondrial ribosomal protein S10
Aliases:   FLJ10567, MRP-S10, PNAS-122
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000048544
Entrez:   55173
Chromosome Mapping:   6p21.1

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