PrimePCR™ Probe Assay: WIPI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WIPI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WIPI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030544
Assay Design:   Intron-spanning
Chromosome Location:   17:66417908-66422280question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000262139 ENST00000546360

Gene Information

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins such as WIPI1 have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al. 2004 [PubMed 15602573]).[supplied by OMIM Mar 2008]

Gene Symbol:   WIPI1
Gene Name:   WD repeat domain, phosphoinositide interacting 1
Aliases:   ATG18, ATG18A, FLJ10055, WIPI49
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000070540
Entrez:   55062
Chromosome Mapping:   17q24.2

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