PrimePCR™ Probe Assay: EN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EN2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030660
Assay Design:   Intron-spanning
Chromosome Location:   7:155251701-155255119question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000297375

Gene Information

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila the 'engrailed' (en) gene plays an important role during development in segmentation where it is required for the formation of posterior compartments. Different mutations in the mouse homologs En1 and En2 produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq Jul 2008]

Gene Symbol:   EN2
Gene Name:   engrailed homeobox 2
Aliases:   Not Available
RefSeq:   NC_000007.13 NG_007124.1 NT_007741.14
Ensembl:   ENSG00000164778
Entrez:   2020
Chromosome Mapping:   7q36

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