PrimePCR™ Probe Assay: SLC30A7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC30A7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC30A7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030800
Assay Design:   Intron-spanning
Chromosome Location:   1:101427423-101431370question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000370112 ENST00000357650 ENST00000370111

Gene Information

Zinc functions as a cofactor for numerous enzymes nuclear factors and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family such as SLC30A7 permit cellular efflux of zinc (Seve et al. 2004 [PubMed 15154973]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC30A7
Gene Name:   solute carrier family 30 (zinc transporter), member 7
Aliases:   DKFZp686M0368, ZNT7, ZnT-7, ZnTL2
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000162695
Entrez:   148867
Chromosome Mapping:   1p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.290000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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