PrimePCR™ Probe Assay: FGA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FGA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FGA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030820
Assay Design:   Intron-spanning
Chromosome Location:   4:155510608-155511835question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000302053 ENST00000403106 ENST00000457487

Gene Information

The protein encoded by this gene is the alpha component of fibrinogen a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading display vasoconstrictor and chemotactic activities and are mitogens for several cell types. Mutations in this gene lead to several disorders including dysfibrinogenemia hypofibrinogenemia afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq Jul 2008]

Gene Symbol:   FGA
Gene Name:   fibrinogen alpha chain
Aliases:   Fib2, MGC119422, MGC119423, MGC119425
RefSeq:   NC_000004.11 NG_008832.1 NT_016354.19
Ensembl:   ENSG00000171560
Entrez:   2243
Chromosome Mapping:   4q28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.970000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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