PrimePCR™ Probe Assay: CA12, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CA12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CA12, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030879
Assay Design:   Intron-spanning
Chromosome Location:   15:63632944-63637686question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000178638 ENST00000344366 ENST00000422263

Gene Information

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes including respiration calcification acid-base balance bone resorption and the formation of aqueous humor cerebrospinal fluid saliva and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues such as kidney colon and pancreas and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CA12
Gene Name:   carbonic anhydrase XII
Aliases:   CAXII, FLJ20151, HsT18816
RefSeq:   NC_000015.9 NG_028022.1 NT_010194.17
Ensembl:   ENSG00000074410
Entrez:   771
Chromosome Mapping:   15q22

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