PrimePCR™ Probe Assay: CA12, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CA12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CA12, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030879
Assay Design:   Intron-spanning
Chromosome Location:   15:63632944-63637686question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000178638 ENST00000344366 ENST00000422263

Gene Information

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes including respiration calcification acid-base balance bone resorption and the formation of aqueous humor cerebrospinal fluid saliva and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues such as kidney colon and pancreas and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CA12
Gene Name:   carbonic anhydrase XII
Aliases:   CAXII, FLJ20151, HsT18816
RefSeq:   NC_000015.9 NG_028022.1 NT_010194.17
Ensembl:   ENSG00000074410
Entrez:   771
Chromosome Mapping:   15q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.870000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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