PrimePCR™ Probe Assay: CHAF1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CHAF1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHAF1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030919
Assay Design:   Intron-spanning
Chromosome Location:   19:4402780-4408982question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000344143 ENST00000535117 ENST00000301280

Gene Information

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50 p60 (CHAF1B; MIM 601245) and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al. 1995 [PubMed 7600578]).[supplied by OMIM Mar 2008]

Gene Symbol:   CHAF1A
Gene Name:   chromatin assembly factor 1, subunit A (p150)
Aliases:   CAF-1, CAF1, CAF1B, CAF1P150, MGC71229, P150
RefSeq:   NC_000019.9 NG_028084.1 NT_011255.14
Ensembl:   ENSG00000167670
Entrez:   10036
Chromosome Mapping:   19p13.3

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