PrimePCR™ Probe Assay: SAFB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SAFB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SAFB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030958
Assay Design:   Intron-spanning
Chromosome Location:   19:5654379-5657295question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000454510 ENST00000540206 ENST00000292123 ENST00000538656 ENST00000433404

Gene Information

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jan 2011]

Gene Symbol:   SAFB
Gene Name:   scaffold attachment factor B
Aliases:   DKFZp779C1727, HAP, HET, SAF-B1, SAFB1
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000160633
Entrez:   6294
Chromosome Mapping:   19p13.3-p13.2

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