This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: LIPE, Human
PrimePCR™ Template for Probe Assay: LIPE, Human
The protein encoded by this gene has a long and a short form generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue among others where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.