PrimePCR™ Probe Assay: CER1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CER1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CER1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031040
Assay Design:   Intron-spanning
Chromosome Location:   9:14720290-14722241question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000380911

Gene Information

This gene encodes a cytokine member of the cysteine knot superfamily characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines together with Dan and DRM/Gremlin represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. [provided by RefSeq Jul 2008]

Gene Symbol:   CER1
Gene Name:   cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
Aliases:   DAND4, MGC119894, MGC119895, MGC96951
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000147869
Entrez:   9350
Chromosome Mapping:   9p23-p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.030000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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