PrimePCR™ Probe Assay: ARID5B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARID5B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARID5B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031143
Assay Design:   Intron-spanning
Chromosome Location:   10:63845573-63850685question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000279873 ENST00000309334

Gene Information

This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   ARID5B
Gene Name:   AT rich interactive domain 5B (MRF1-like)
Aliases:   DESRT, FLJ21150, MRF2
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000150347
Entrez:   84159
Chromosome Mapping:   10q21.2

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