PrimePCR™ Probe Assay: CCNA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031254
Assay Design:   Intron-spanning
Chromosome Location:   4:122742135-122743624question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000274026

Gene Information

The protein encoded by this gene belongs to the highly conserved cyclin family whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1 which is present only in germ cells this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq Jul 2008]

Gene Symbol:   CCNA2
Gene Name:   cyclin A2
Aliases:   CCN1, CCNA
RefSeq:   NC_000004.11 NG_009111.1 NT_016354.19
Ensembl:   ENSG00000145386
Entrez:   890
Chromosome Mapping:   4q25-q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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