PrimePCR™ Probe Assay: CBLN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CBLN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CBLN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031341
Assay Design:   Intron-spanning
Chromosome Location:   16:49313389-49314873question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000219197 ENST00000536749

Gene Information

This gene encodes a cerebellum-specific precursor protein precerebellin with similarity to the globular (non-collagen-like) domain of complement component C1qB. Precerebellin is processed to give rise to several derivatives including the hexadecapeptide cerebellin which is highly enriched in postsynaptic structures of Purkinje cells. Cerebellin has also been found in human and rat adrenals where it has been shown to enhance the secretory activity of this gland. [provided by RefSeq Aug 2008]

Gene Symbol:   CBLN1
Gene Name:   cerebellin 1 precursor
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000102924
Entrez:   869
Chromosome Mapping:   16q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.480000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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