PrimePCR™ Probe Assay: EIF2AK3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EIF2AK3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF2AK3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031378
Assay Design:   Intron-spanning
Chromosome Location:   2:88890519-88892912question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000419748 ENST00000303236 ENST00000415570 ENST00000535951

Gene Information

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2) leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER) where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq Jan 2010]

Gene Symbol:   EIF2AK3
Gene Name:   eukaryotic translation initiation factor 2-alpha kinase 3
Aliases:   DKFZp781H1925, PEK, PERK, WRS
RefSeq:   NC_000002.11 NG_016424.1 NT_022184.15
Ensembl:   ENSG00000172071
Entrez:   9451
Chromosome Mapping:   2p12

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