This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: KDM4A, Human
PrimePCR™ Template for Probe Assay: KDM4A, Human
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain a JmjC domain a JD2H domain two TUDOR domains and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase converting specific trimethylated histone residues to the dimethylated form and as a transcriptional repressor. [provided by RefSeq Apr 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.