PrimePCR™ Probe Assay: KDM4A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KDM4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KDM4A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031398
Assay Design:   Intron-spanning
Chromosome Location:   1:44125971-44128612question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000372396

Gene Information

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain a JmjC domain a JD2H domain two TUDOR domains and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase converting specific trimethylated histone residues to the dimethylated form and as a transcriptional repressor. [provided by RefSeq Apr 2009]

Gene Symbol:   KDM4A
Gene Name:   lysine (K)-specific demethylase 4A
Aliases:   JHDM3A, JMJD2, JMJD2A, KIAA0677
RefSeq:   NC_000001.10 NG_028196.1 NT_032977.9
Ensembl:   ENSG00000066135
Entrez:   9682
Chromosome Mapping:   1p34.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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