PrimePCR™ Probe Assay: KDM4C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KDM4C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KDM4C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031414
Assay Design:   Intron-spanning
Chromosome Location:   9:6880037-6888037question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000381309 ENST00000381306 ENST00000535193 ENST00000543771 ENST00000442236 ENST00000536108

Gene Information

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain one JmjN domain two PHD-type zinc fingers and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2009]

Gene Symbol:   KDM4C
Gene Name:   lysine (K)-specific demethylase 4C
Aliases:   FLJ25949, GASC1, JHDM3C, JMJD2C, KIAA0780, bA146B14.1
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000107077
Entrez:   23081
Chromosome Mapping:   9p24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.910000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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