PrimePCR™ Probe Assay: CLTC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $220.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031476
Assay Design:   Intron-spanning
Chromosome Location:   17:57697444-57721706question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000269122 ENST00000393043

Gene Information

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq Jul 2008]

Gene Symbol:   CLTC
Gene Name:   clathrin, heavy chain (Hc)
Aliases:   CHC, CHC17, CLH-17, CLTCL2, Hc, KIAA0034
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000141367
Entrez:   1213
Chromosome Mapping:   17q11-qter

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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