PrimePCR™ Probe Assay: AFP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AFP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AFP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031540
Assay Design:   Intron-spanning
Chromosome Location:   4:74319524-74321373question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000395792

Gene Information

This gene encodes alpha-fetoprotein a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms and binds copper nickel fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq Jul 2008]

Gene Symbol:   AFP
Gene Name:   alpha-fetoprotein
Aliases:   FETA, HPAFP
RefSeq:   NC_000004.11 NG_023028.1 NT_022778.16
Ensembl:   ENSG00000081051
Entrez:   174
Chromosome Mapping:   4q11-q13

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