PrimePCR™ Probe Assay: SGMS2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SGMS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SGMS2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031554
Assay Design:   Intron-spanning
Chromosome Location:   4:108820749-108824462question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000394684 ENST00000503862 ENST00000359079 ENST00000394686

Gene Information

Sphingomyelin a major component of cell and Golgi membranes is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants but the full-length nature of their transcripts has not been determined.[provided by RefSeq Oct 2008]

Gene Symbol:   SGMS2
Gene Name:   sphingomyelin synthase 2
Aliases:   MGC26963, SMS2
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164023
Entrez:   166929
Chromosome Mapping:   4q25

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