PrimePCR™ Probe Assay: ROCK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ROCK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ROCK2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031597
Assay Design:   Intron-spanning
Chromosome Location:   2:11364468-11367432question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000315872 ENST00000401753 ENST00000431087

Gene Information

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis smooth muscle contraction the formation of actin stress fibers and focal adhesions and the activation of the c-fos serum response element. This protein which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq Jul 2008]

Gene Symbol:   ROCK2
Gene Name:   Rho-associated, coiled-coil containing protein kinase 2
Aliases:   KIAA0619
RefSeq:   NC_000002.11 NT_005334.16
Ensembl:   ENSG00000134318
Entrez:   9475
Chromosome Mapping:   2p24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.340000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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