PrimePCR™ Probe Assay: DOCK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DOCK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DOCK2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031654
Assay Design:   Intron-spanning
Chromosome Location:   5:169230124-169267794question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000256935 ENST00000520908 ENST00000519628 ENST00000343291 ENST00000540750

Gene Information

The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells predominantly in the peripheral blood leukocytes and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq Oct 2011]

Gene Symbol:   DOCK2
Gene Name:   dedicator of cytokinesis 2
Aliases:   FLJ46592, KIAA0209
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000134516
Entrez:   1794
Chromosome Mapping:   5q35.1

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